Multispecies microbial communities or bacteria grown with eukaryotic cells can be to hard grow and measure. The thermograms produced during microcalorimetry experiments are so unique and high resolution that they can be used to monitor individual components of a complex model in real-time. This can be done without the need for labeling or destructive sampling.
See individual components
With our analysis methods, it is is possible to see the contribution of each component to the complex model.
Simple to set up
Complex model systems are hard enough to set up and monitor so we make things easier with the calScreener™. Simply add your sample to the plate and we take care of the rest.
No labeling needed
The thermograms produced by the calScreener™ to not require any labeling or special pre-treatment.
If you need to do more analysis on your sample after the experiment is over, the sample will be completely intact making complementary assays possible.
Get new information
Whatever your model system, microcalorimetry can give you new information and help you understand it better.
Complex drug treatment assays
See how drugs respond under more realistic conditions. For example, the calScreener™ can show the effect of antibiotics on microbes grown together with eukaryotic cells.
Data courtesy of Dr. Mattias Svensson and Dr. Anna Norrby-Teglund laboratory,
Here the graphs show two different complex models (lung and skin) and how the heat flow changes in response to the addition of Staphyloccus aurues. In both cases, the tissue model alone produces relatively low and consistent heat flow over time. However, upon the addition of S. aureus, the metabolism of the models changes dramatically as seen by the sharp increase in heat flow.
In the lung model, the heat flow increases sharply and gradually starts to decrease at around 6 hours.
In the skin model, there is a peak of metabolic activity with a sharp increase followed by a sharp decease between 5 and 10 hours. Following the peak, the heat flow decreases gradually over time.
These figures show that the metabolism of complex models is greatly impacted by the addition of bacteria to simulate an infection. There are currently no other methods to visualise the infection process in this way. These graphs demonstrate the exciting possibility to study treatments that can be applied to complex models and visualised in real time.
Whether you work on multispecies biofilms or complex organoids, microcalorimetry can give you new information into your model system. You can detect how individual components of the system respond to experimental treatments, such as the addition of antimicrobials. Image an intestinal organoid cultured with bacteria and exposed to clinical doses of antibiotics. Using microcalorimetry, it would be possible to monitor the effect of the antimicrobial in real time and determine if the composition change after the antibiotic is removed and how does the epithelial lining respond. All of this can be done with a simple, non-invasive assay.
The process could not be simpler. Add the organoid to a well in our calScreener™ plate and away you go. There is no need for labeling or continuous sampling to get high resolution real-time data.