In the present study, we used for the first time, a metabolic approach to detect the presence of Candida in peritoneal fluid samples. The study was registered with ClinicalTrials.gov (ID number 05264571). All experiments were performed at the laboratory of mycology of the university hospital of Nancy. The metabolic evaluation used the calScreener™ technology from Symcel AB (Sweden) [6,7]. The calScreener™ measures the heatflow from living cells in real time. The device is based on isothermal calorimetry. It can be used on different types of microorganisms and in different types of media, including blood. The calScreener™ provides real-time data over a prolonged time period. Basically, after stabilization of the signal, a lag phase (plateau) occurs before the metabolic activity raises. Then the metabolic activity reaches a peak followed by a decline. Different parameters can be evaluated directly from the curve such as time to activity. Each specific pathogen gives rise to a unique growth pattern in the kinetic data, which allows its identification. Presently, available data pertains to bacteria [8,9], and there is a lack of kinetic data for Candida. The technology uses cell growth plates: 48 well micro titer cell culture plates with 32 biological samples and 16 thermodynamic internal reference position. Samples were referral and clinical strains of C. albicans and C. glabrata. Referral strains were ATCC90028 for C. albicans and ATCC66032 for C. glabrata. Media were Sabouraud(SBD), ascitic(AF), and peritoneal fluid(PF) issued from a biological collection (the pBDG2 study, NCT03997929, which enrolled patients with suspected or confirmed intra-abdominal infections). All selected samples were pathogen-free using a microbiological analysis (direct examination and culture) to look for the presence of bacteria and fungi, to avoid contamination.