This application note outlines the use of the Symcel calScreener™ in combination with Synthetic CF Sputum Media (SCFM) to model cystic fibrosis-related biofilms. This approach provides real-time, in situ analysis of biofilm kinetics, offering insights into treatment efficacy under clinically relevant conditions, and addressing limitations of conventional biofilm models.

This study explores the use of isothermal microcalorimetry to detect bacterial spoilage in minced chicken meat. The method allows for real-time, direct measurement of microbial metabolic activity, distinguishing between fresh and spoiled meat based on microbial load. The findings demonstrate the sensitivity of this approach, including the detection of low-level contamination by Salmonella enterica.

This study presents the use of isothermal microcalorimetry for optimizing growth media in probiotic development. By continuously measuring metabolic heat flow, this approach allows real-time assessment of microbial activity under various media conditions, offering insights into growth rates and metabolic yield. The method provides an efficient and precise alternative to traditional biomass-focused techniques, facilitating the development of cost-effective, optimized media formulations.

This study highlights the use of isothermal microcalorimetry to evaluate antimicrobial coatings and materials. By measuring real-time heat flow associated with bacterial metabolism, this method provides insights into bacterial attachment, antimicrobial compound leaching, and contact-dependent bacterial killing. The technique offers a precise and efficient approach to assessing the antimicrobial properties of materials under conditions that simulate real-world applications, such as medical devices and industrial surfaces.